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buffer against phospho akt ser473  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc buffer against phospho akt ser473
    Buffer Against Phospho Akt Ser473, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 17211 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/buffer+against+phospho+akt+ser473/Phospho-Akt+(Ser473)+XP+Rabbit+mAb/pm33665823-74-11-14
    Average 99 stars, based on 17211 article reviews
    buffer against phospho akt ser473 - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Western Blot:

    Article Title: Cardiac glycosides cause cytotoxicity in human macrophages and ameliorate white adipose tissue homeostasis.
    Article Snippet: A total of 50 μg protein was diluted 1:1 with 2× Laemmli buffer (BioRad), resolved by SDS-PAGE,transferred into PVDF membranes (0.2 um pore size) and blocked with 5% bovine serum albumin (Sigma). .. These were subjected to immunoblotting with the primary antibodies in blocking buffer against phospho-Akt-Ser473 (Cell Signaling Technology Cat# 4060, RRID:AB_2315049), panAkt (Cell Signaling Technology Cat# 4691, RRID:AB_915783), CD206 (Bio-Rad Cat# MCA2155T, RRID:AB_2144910), CD36 (Thermo Fisher Scientific Cat# PA1-16813, RRID:AB_568487), ACTB (Santa Cruz Biotechnology Cat# sc-47778, RRID:AB_626632) and HRP-labelled secondary antibodies (Thermo Fisher Scientific Cat# 31464, RRID:AB_228378 and Cat# 31450, RRID:AB_228427). .. The probed proteins were detected using SuperSignal West Femto Chemiluminescent Substrate (Thermo Fisher).

    Blocking Assay:

    Article Title: Cardiac glycosides cause cytotoxicity in human macrophages and ameliorate white adipose tissue homeostasis.
    Article Snippet: A total of 50 μg protein was diluted 1:1 with 2× Laemmli buffer (BioRad), resolved by SDS-PAGE,transferred into PVDF membranes (0.2 um pore size) and blocked with 5% bovine serum albumin (Sigma). .. These were subjected to immunoblotting with the primary antibodies in blocking buffer against phospho-Akt-Ser473 (Cell Signaling Technology Cat# 4060, RRID:AB_2315049), panAkt (Cell Signaling Technology Cat# 4691, RRID:AB_915783), CD206 (Bio-Rad Cat# MCA2155T, RRID:AB_2144910), CD36 (Thermo Fisher Scientific Cat# PA1-16813, RRID:AB_568487), ACTB (Santa Cruz Biotechnology Cat# sc-47778, RRID:AB_626632) and HRP-labelled secondary antibodies (Thermo Fisher Scientific Cat# 31464, RRID:AB_228378 and Cat# 31450, RRID:AB_228427). .. The probed proteins were detected using SuperSignal West Femto Chemiluminescent Substrate (Thermo Fisher).



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    99
    Cell Signaling Technology Inc buffer against phospho akt ser473
    Buffer Against Phospho Akt Ser473, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/buffer+against+phospho+akt+ser473/Phospho-Akt+(Ser473)+XP+Rabbit+mAb/pm33665823-74-11-14
    Average 99 stars, based on 1 article reviews
    buffer against phospho akt ser473 - by Bioz Stars, 2026-09
    99/100 stars
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